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KMID : 0380620080400020123
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Korean Journal of Food Science and Technology
2008 Volume.40 No. 2 p.123 ~ p.128
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Chemistry/Analysis : Development of a Simultaneous Analysis Method for DDT (DDD & DDE) in Ginseng
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Kim Sung-Dan
Cho Tae-Hee
Han Eun-Jung
Park Seoung-Gyu
Han Chang-Ho
Jo Han-Bin
Choi Byung-Hyun
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Abstract
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The MRLs (maximum residue limits) of DDT (DDD and DDE) in fresh ginseng, dried ginseng, and steamed red ginseng are set as low as 0.01 mg/kg, 0.05 mg/kg, and 0.05 mg/kg, respectively. Therefore, this study was undertaken to develop a simple and highly sensitive analysis method, as well as to reduce interfering ginseng matrix peaks, for the determination of DDT isomers (o,p`-DDE, p,p`-DDE, o,p`-DDD, p,p`-DDD, o,p`-DDT, and p,p`-DDT) in fresh ginseng, dried ginseng, and steamed red ginseng at the 0.01 mg/kg level. The method used acetonitrile extraction according to simultaneous analysis, followed by normal-phase Florisil solid-phase extraction column clean-up. The purification method entailed the following steps: (1) dissolve the concentrated sample extract in 7 mL hexane; (2) add 3 mL of H2SO4; (3) vigorously shake on a vortex mixer; (4) cetrifuge at 2000 rpm for 5 min; (5) transfer 3.5 mL of the supernatant to the Florisil-SPE (500 mg/6 mL); and (6) elute the SPE column with 1.5 mL of hexane and 10 mL of ether/hexane (6:94). The determination of DDT isomers was carried out by a gas chromatography-electron capture detector (GC-μECD). The hexane and ether/hexane (6:94) eluate significantly removed chromatographic interferences, and the addition of 30% H2SO4 to the acetonitrile extract effectively reduced many interfering ginseng matrix peaks, to allow for the determination of the DDT isomers at the 0.01 mg/kg level. The recoveries of the 6 fortified (most at 0.01 mg/kg) DDT isomers from fresh ginseng, dried ginseng, and steamed red ginseng ranged from 87.9 to 99.6%. The MDLs (method detection limits) ranged from 0.003 to 0.009 mg/kg. Finally, the application of this method for the determination of DDT isomers is sensitive, rapid, simple, and inexpensive.
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KEYWORD
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DDT (DDD & DDE), ginseng, simultaneous analysis
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